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anti mouse cd206 antibody  (Elabscience Biotechnology)


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    Elabscience Biotechnology anti mouse cd206 antibody
    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of <t>CD206</t> (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
    Anti Mouse Cd206 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+mouse+cd206+mmr+antibody/PE+Anti-Mouse+CD206%2FMMR+Antibody/pmc13091142-309-8-11
    Average 95 stars, based on 60 article reviews
    anti mouse cd206 antibody - by Bioz Stars, 2026-09
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    1) Product Images from "Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration"

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.04.004

    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
    Figure Legend Snippet: LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Techniques Used: Activation Assay, Flow Cytometry, RNA Sequencing, Marker, Expressing, Injection, Immunofluorescence, Co-Culture Assay, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Marker:

    Article Title: Tumor-intrinsic ENO1 inhibition promotes antitumor immune response and facilitates the efficacy of anti-PD-L1 immunotherapy in bladder cancer.
    Article Snippet: These suspensions were then treated with Fcblock (Elabscience, catalog no. E-AB-F0997A) to inhibit nonspecific Fc-receptor-mediated binding before staining. .. Marker staining was performed using fixable viability dye (BD Pharmingen, catalog no. 565388), Percp/ Cyanine 5.5-conjugated anti-mouse CD45 (Elabscience, E-AB-F1136J), APC-conjugated anti-mouse CD3 (Elabscience, E-AB-F1013E), or PE-conjugated anti-mouse CD8 (Elabscience, E-AB-F1104D), PE-conjugated antimouse GZMB (ThermoFisher Scientific, 12-8898-82),PEconjugated anti-mouse IFN-(Elabscience, E-AB-F1101D), FITC-conjugated anti-mouse GZMB (ThermoFisher Scientific, 11-8898-82), FITC-conjugated anti-mouse IFN- (Elabscience, E-AB-F1101C), FITC Anti-Mouse/ Human CD11b Antibody (Elabscience, E-AB-F1081C), PE anti-Mouse CD206/MMR antibody (Elabscience, E-AB-F1135D), or APC anti-Mouse CD86 antibody (Elabscience, E-AB-F0994E) on ice in the dark for 40min. .. Subsequently, the results were analyzed using a flow cytometer (CytoFLEX S; Beckman Coulter), with data processed using CytExpert software.

    Article Title: Tumor-intrinsic ENO1 inhibition promotes antitumor immune response and facilitates the efficacy of anti-PD-L1 immunotherapy in bladder cancer
    Article Snippet: These suspensions were then treated with Fc-block (Elabscience, catalog no. E-AB-F0997A) to inhibit nonspecific Fc-receptor-mediated binding before staining. .. Marker staining was performed using fixable viability dye (BD Pharmingen, catalog no. 565388), Percp/Cyanine 5.5-conjugated anti-mouse CD45 (Elabscience, E-AB-F1136J), APC-conjugated anti-mouse CD3 (Elabscience, E-AB-F1013E), or PE-conjugated anti-mouse CD8 (Elabscience, E-AB-F1104D), PE-conjugated anti-mouse GZMB (ThermoFisher Scientific, 12-8898-82),PE-conjugated anti-mouse IFN-(Elabscience, E-AB-F1101D), FITC-conjugated anti-mouse GZMB (ThermoFisher Scientific, 11-8898-82), FITC-conjugated anti-mouse IFN- (Elabscience, E-AB-F1101C), FITC Anti-Mouse/Human CD11b Antibody (Elabscience, E-AB-F1081C), PE anti-Mouse CD206/MMR antibody (Elabscience, E-AB-F1135D), or APC anti-Mouse CD86 antibody (Elabscience, E-AB-F0994E) on ice in the dark for 40min. .. Subsequently, the results were analyzed using a flow cytometer (CytoFLEX S; Beckman Coulter), with data processed using CytExpert software.

    Staining:

    Article Title: Tumor-intrinsic ENO1 inhibition promotes antitumor immune response and facilitates the efficacy of anti-PD-L1 immunotherapy in bladder cancer.
    Article Snippet: These suspensions were then treated with Fcblock (Elabscience, catalog no. E-AB-F0997A) to inhibit nonspecific Fc-receptor-mediated binding before staining. .. Marker staining was performed using fixable viability dye (BD Pharmingen, catalog no. 565388), Percp/ Cyanine 5.5-conjugated anti-mouse CD45 (Elabscience, E-AB-F1136J), APC-conjugated anti-mouse CD3 (Elabscience, E-AB-F1013E), or PE-conjugated anti-mouse CD8 (Elabscience, E-AB-F1104D), PE-conjugated antimouse GZMB (ThermoFisher Scientific, 12-8898-82),PEconjugated anti-mouse IFN-(Elabscience, E-AB-F1101D), FITC-conjugated anti-mouse GZMB (ThermoFisher Scientific, 11-8898-82), FITC-conjugated anti-mouse IFN- (Elabscience, E-AB-F1101C), FITC Anti-Mouse/ Human CD11b Antibody (Elabscience, E-AB-F1081C), PE anti-Mouse CD206/MMR antibody (Elabscience, E-AB-F1135D), or APC anti-Mouse CD86 antibody (Elabscience, E-AB-F0994E) on ice in the dark for 40min. .. Subsequently, the results were analyzed using a flow cytometer (CytoFLEX S; Beckman Coulter), with data processed using CytExpert software.

    Article Title: Tumor-intrinsic ENO1 inhibition promotes antitumor immune response and facilitates the efficacy of anti-PD-L1 immunotherapy in bladder cancer
    Article Snippet: These suspensions were then treated with Fc-block (Elabscience, catalog no. E-AB-F0997A) to inhibit nonspecific Fc-receptor-mediated binding before staining. .. Marker staining was performed using fixable viability dye (BD Pharmingen, catalog no. 565388), Percp/Cyanine 5.5-conjugated anti-mouse CD45 (Elabscience, E-AB-F1136J), APC-conjugated anti-mouse CD3 (Elabscience, E-AB-F1013E), or PE-conjugated anti-mouse CD8 (Elabscience, E-AB-F1104D), PE-conjugated anti-mouse GZMB (ThermoFisher Scientific, 12-8898-82),PE-conjugated anti-mouse IFN-(Elabscience, E-AB-F1101D), FITC-conjugated anti-mouse GZMB (ThermoFisher Scientific, 11-8898-82), FITC-conjugated anti-mouse IFN- (Elabscience, E-AB-F1101C), FITC Anti-Mouse/Human CD11b Antibody (Elabscience, E-AB-F1081C), PE anti-Mouse CD206/MMR antibody (Elabscience, E-AB-F1135D), or APC anti-Mouse CD86 antibody (Elabscience, E-AB-F0994E) on ice in the dark for 40min. .. Subsequently, the results were analyzed using a flow cytometer (CytoFLEX S; Beckman Coulter), with data processed using CytExpert software.

    Incubation:

    Article Title: SESN3 restrains the progress of idiopathic pulmonary fibrosis by targeting the activity of FOSL2.
    Article Snippet: .. After fixing and permeabilizing, cells were incubated with PE Anti-Mouse CD206/MMR antibody (Elabscience, Wuhan, China) for 30 min. .. The treated cells were washed and resuspended in dye buffer and read by a flow cytometer (Agilent, Santa Clara, CA, USA).

    Article Title: SESN3 restrains the progress of idiopathic pulmonary fibrosis by targeting the activity of FOSL2
    Article Snippet: .. After fixing and permeabilizing, cells were incubated with PE Anti-Mouse CD206/MMR antibody (Elabscience, Wuhan, China) for 30 min. .. The treated cells were washed and resuspended in dye buffer and read by a flow cytometer (Agilent, Santa Clara, CA, USA).

    Fluorescence:

    Article Title: Intranasal blood-brain barrier bypass enables sequential mitochondria-targeted bioengineered nanolamellar system for ischemic stroke therapy
    Article Snippet: ROS detection kit was purchased from Biosharp Biotechnology Co., Ltd. (Beijing, China). .. The fluorescence-labelled antibodies used for immunocyte infiltrations: Elab Fluor® Violet 450 Anti-Mouse CD45 Antibody (Elabscience, E-AB-F1136Q, FC, not diluted); APC Anti-Mouse/Human CD11b Antibody (Elabscience, E-AB-F1081E, FC, not diluted); FITC Anti-Mouse F4/80 Antibody (Elabscience, E-AB-F0995C, FC, not diluted); PE/Cyanine7 Anti-Mouse CD86 Antibody (Elabscience, E-AB-F0994H, FC, not diluted); PE Anti-Mouse CD206/MMR Antibody (Elabscience, E-AB-F1135D, FC, not diluted). ..



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    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of <t>CD206</t> (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
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    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of <t>CD206</t> (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
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    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Journal: Bioactive Materials

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    doi: 10.1016/j.bioactmat.2026.04.004

    Figure Lengend Snippet: LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Article Snippet: Following permeabilization, cells were stained with Phycoerythrin (PE)-conjugated anti-mouse CD206 antibody (Elabscience, E-AB-F1135D) for 30 min at 4 °C in the dark.

    Techniques: Activation Assay, Flow Cytometry, RNA Sequencing, Marker, Expressing, Injection, Immunofluorescence, Co-Culture Assay, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay